Growth of Streptomyces aureofaciens in Continuous Culture

Genetic Recombination in Crosses Between Streptomyces aureofaciens and Streptomyces rimosus.

Polsinelli, M. (University of Pavia, Pavia, Italy), and Maria Beretta. Genetic recombination in crosses between Streptomyces aureofaciens and Streptomyces rimosus. J. Bacteriol. 91:63-68. 1966.-Biochemical mutants were obtained from Streptomyces rimosus and S. aureofaciens by ultraviolet irradiation. Crosses were performed between auxotrophic strains of S. rimosus and S. aureofaciens with posit...

Ribonuclease from Streptomyces aureofaciens at atomic resolution.

Crystals of ribonuclease from Streptomyces aureofaciens diffract to atomic resolution at room temperature. Using synchrotron radiation and an imaging-plate scanner, X-ray data have been recorded to 1.20 A resolution from a crystal of native enzyme and to 1.15 A from a crystal of a complex with guanosine-2'-monophosphate. Refinement with anisotropic atomic temperature factors resulted in increas...

Metabolic Behavior and Chlortetracycline Production by Streptomyces aureofaciens in Liquid Culture.

Standard fermentations were performed as follows: A spore suspension was inoculated into 3 L of the following medium: sucrose, 3 per cent; corn steep liquor, 1 per cent; molasses, 0.1 per cent; NaCl, 0.05 per cent; and CaCO3, 0.4 per cent. The fermentations were carried out at 27 C in 5-L fermentors (Bartholomew, Karow and Sfat, 1950). After 36 hours, 100 ml of the culture fluid was transferred...

Production of tetracycline by Streptomyces aureofaciens in synthetic media.

Genome Sequence of Streptomyces aureofaciens ATCC Strain 10762

Streptomyces aureofaciens is a Gram-positive actinomycete that produces the antibiotics tetracycline and chlortetracycline. Here, we report the assembly and initial annotation of the draft genome sequence of S. aureofaciens ATCC strain 10762.